Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride

Surface modification of biomaterials is a promising approach to control biofunctionality while retaining the bulk biomaterial properties. Perlecan major proteoglycan in vascular basement membrane that supports low levels platelet adhesion but not activation. Thus, perlecan bioactive for blood-contacting applications. This study furthers mechanistic understanding interactions with by establishing platelets utilize domains III and V core protein adhesion. Polyvinyl chloride (PVC) functionalized recombinant human domain (rDV) explore effect tethering method on orientation bioactivity. Tethering rDV PVC achieved via either physisorption or covalent attachment plasma immersion ion implantation (PIII) treatment. Both methods reduce activation compared pristine PVC, however, mechanisms are unique each method. Physisorption orientates molecule hinder access integrin-binding region, which inhibits In contrast, PIII treatment allow rendered antiadhesive glycosaminoglycan (GAG) chain. These effects demonstrate potential biofunctionalization modulate blood contacting